Research Assistant I
TLDR
Adapt extremophile microorganisms to Mars-relevant conditions using mutagenesis and low-pressure simulation chambers.
The Costes Laboratory at the Trivedi Institute for space and global biomedicine is seeking a Research Intern I to support the First Martian project, an effort focused on adapting extremophile organisms to survive Mars-relevant environmental conditions, including low atmospheric pressure, low temperature and hypoxic environments. Our lab employs methods from microbiology, molecular biology, environmental simulation coupled with multi-omics and high thruput imaging to study how organisms respond and adapt to extreme, planetary-analog stress conditions.
Job Summary
Performs laboratory culturing, maintenance, and experimental manipulation of extremophile microorganisms, including mutagenesis procedures aimed at adapting organisms to low-pressure and low-temperature environments. Operates and maintains laboratory and environmental simulation equipment, and assists with inventory and ordering.
Essential Functions
• Culture and maintain extremophile microorganism strains under sterile technique.
• Perform mutagenesis procedures and selection experiments to adapt organisms to reduced pressure and reduced temperature conditions.
• Operate and monitor benchtop low-pressure/gas-composition simulation chambers, including pressure and atmosphere control systems.
• Prepare and test experimental samples across varying pressure, temperature, and gas composition conditions.
• Operate, maintain, and sterilize laboratory equipment; maintain general lab cleanliness.
• Keep detailed and accurate experimental data, logbooks, and protocols.
• Organize, document, and help analyze experimental results.
• Maintain inventory and assist with ordering of lab supplies, reagents, and gases.
• Follow all lab safety protocols, including safe handling of compressed gas cylinders and vacuum equipment.
Physical Effort
Moderate physical effort required. May be required to stand/move for several hours at a time. May be required to move objects up to 50 pounds (e.g., gas cylinders, equipment) with or without the assistance of equipment.
Currently enrolled in or recently completed a Bachelor's degree program in Microbiology, Biology, Biomedical Engineering, or related field required.
No experience required; prior lab/microbiology experience preferred.
The University of Pittsburgh is an equal opportunity employer / disability / veteran.
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