eGenesis

Research Associate II/Senior Research Associate, Analytical Development

Cambridge, Massachusetts, U.S.

Full-Time

On-site

$80,000 – $98,000 per year

TLDR

Develop and qualify assays for transgene expression in genetically modified porcine cells using primary cell culture, flow cytometry, ELISA, and PCR.

About eGenesis

eGenesis is a clinical-stage biotechnology company developing human-compatible engineered organs to address the severe global organ shortage. The Company’s proprietary genome engineering platform enables extensive, multiplex gene edits to remove key biological barriers, add protective human transgenes, and inactivate endogenous retroviruses. EGEN-2784, a genetically engineered porcine kidney, is the Company’s lead program and is currently being evaluated in a multi-patient Expanded Access study at MGH. eGenesis is headquartered in Cambridge, MA.

POSITION SUMMARY

We are seeking a highly motivated individual to join the Analytical Development team as a Research Associate II / Senior Research Associate. This position is an excellent opportunity to help develop new gene editing and xenotransplantation products in an exciting and dynamic environment focused on cutting-edge molecular and cellular engineering technology.

In this role, you will be part of a small team responsible for developing and qualifying methods to assess transgene expression and function in genetically modified porcine cells. This role will involve significant cell culture work, including the isolation, growth and maintenance of primary cells, such as endothelial cells from various porcine organs, and characterizing these cells using techniques such as flow cytometry, ELISA, and PCR. To be successful in this role, you will leverage previous experience with immunological and genomic assays. This role is a full-time lab-based position in which being proactive, determined, responsible, coachable, and collaborative will help you thrive.

PRIMARY RESPONSIBILITIES

Independently plan development, optimization, troubleshooting, and execution of robust and reproducible analytical assays to characterize genetically edited porcine animals

Utilize various techniques to test the primary cells and/or factors they produce through routine analytical testing (including flow cytometry, ELISA, conventional and real-time PCR, etc)

Proactively monitor, track, and bridge critical reagent inventory (e.g., cell lines, assay standards, antibodies) to maintain continuous laboratory workflow without supply gaps or delays

Independently identify assay anomalies or failures, conduct root-cause analyses, and propose paths forward to drive group goals to timely completion

Optimize laboratory time and material resources through disciplined sample batching and full-plate assay executions

Partner closely with QC to lead and support internal technology transfers of analytical methods, ensuring seamless assay transitions, robustness and cross-functional alignment

Communicate and present scientific findings at group and company meetings

Consistently document experimental procedures and data in ELNs within standard departmental turnaround timelines

Contribute to multiple research projects as they arise in our fast-paced, start-up environment

Build our laboratory efforts by maintaining and adding to a productive and fun environment

QUALIFICATIONS

B.Sc with at least 4 years of industry experience in cell biology, immunology, biomedical engineering or related discipline

Prior experience in development, troubleshooting and routine execution of assays to characterize and measure transgene expression and potency, using a variety of methodologies, particularly flow cytometry and ELISA

Proficiency in growing and maintaining mammalian primary cells/cell lines in culture and aseptic cell culture techniques

Expertise in general cellular and molecular biology techniques, including ELISA, flow cytometry (FACS), and potency assays 

Demonstrated ability to self-manage daily schedules, independently prioritize tasks across multiple concurrent projects, and adapt efficiently in a fast-paced environment

Proven track record of optimizing lab efficiency, including sample batching, material resource management, and proactive inventory tracking systems.

Strong interpersonal skills and ability to work in teams

The base salary range for this position is $80,000 - $98,000.

eGenesis

eGenesis develops innovative genome engineering technologies aimed at providing safe and effective organ solutions for patients facing organ failure. Our focus is on creating alternatives that can make organ transplants more accessible and reliable, significantly improving patients' quality of life.

Founded: Founded 2015
Employees: 51-200 employees
Total raised: $140M raised

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Research Associate